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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
详见说明书
- 供应商:
广州华韵生物科技有限公司,www.huayunbio.cn
- 规格:
10次
从动物组织/培养细胞中提取总DNA
产品简介:
HiPure Tissue DNA Mini Kit采用快速的硅胶柱纯化技术,简化了DNA抽提流程。整个过程无需酚氯仿抽提和耗时的醇类沉淀。HiPure Tissue DNA Mini Kit适合于从1-30mg动物组织和小于10*7个细胞中提取高纯度的基因组DNA和线粒体DNA。纯化的DNA可直接于PCR,酶切和Southern杂交。
| 产品特性与优点: 重复性好 - 适合于从不同类型的组织样品中提取高产量DNA 高纯度DNA - 用于敏感的下游应用, 如多重及定量 PCR 快速 - 简化的流程,可在20分钟内完成数个样品的提取工作(消化后) 安全 - 无需酚氯仿抽提,无需醇类沉淀 |
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
(Blumenthal and Landers 1973. BBRC 55:680-688). Therefore, the most critical factor is getting the RNA in contact with ATA before nucleases can bind to the nucleic acid and degrade it. Supported by federal grants to J.J.L. and J.C.D. Fig. 1. ATA mini
Mini DNA extraction from wheat leaves for PCR and Southern B
Procedure for 100 ~ 300 mg of tissue. 1. Collect fresh tissue, coleoptiles or small amount of leaf tissue. Use about three inches of a leaf blade. Preferably younger tissue. Fold leaf and place in a 2 ml microfuge tube. 2. Grind in liquid
-throughput purification of genomic DNA and total RNA from up to 96 samples. DNA and RNA are simultaneously purified from each cell or tissue sample. Since there is no need to divide each sample into two for separate purification procedures, maximum yields
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