- ￥2940
- 华韵生物(Huayunbio)
- HYPC5138
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州华韵生物科技有限公司
- 规格：
10µg
产品信息
| 别名 | Fibroblast Growth Factor 12; FGF-12; Fibroblast Growth Factor Homologous Factor 1; FHF-1; Myocyte-Activating Factor; FGF12; FGF12B; FHF1 |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | Met1-Thr181 |
| 检索号 | P61328-2 |
| 分子量 | 20.5 kDa |
| 表观分子量 | 19 kDa |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2~8℃ for 2~7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS, 5mM EDTA, pH 7.5. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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文献和实验Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
at 200 x g for 4 minutes. Remove the supernatant and gently flick the pellet. Resuspend the pellet in an appropriate amount of Human Feeder Cell Conditioned Medium supplemented with 4 ng/mL of recombinant human FGF basic. Add
Human FastTrack mRNA Isolation
. 8.Incubate for 2 minutes at room temperature. Vortex the sample to resuspend the cellulose completely. 9.Rock the tube gently in a horizontal position for 60-90 minutes at room temperature. 10.Centrifuge the oligo(dT) slurry at 3,000 x g for 5 minutes
Clonality - X Chromosome Inactivation Assay
of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgen-receptor gene correlates with X chromosome inactivation. Am J Hum Genet 51:1229-39, 1992 . Enomoto T, Fujita M, Inoue M, Tanzawa O, Nomura T, Shroyer K. Analysis
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