- ￥5340
- 华韵生物(Huayunbio)
- HYPC5246
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州华韵生物科技有限公司
- 规格：
10µg
产品信息
| 别名 | Interleukin-10; Il10; IL-10; Cytokine synthesis inhibitory factor; CSIF |
| 物种 | Mouse |
| 表达宿主 | E.coli |
| 序列信息 | Ser19-Ser178 |
| 检索号 | P18893 |
| 分子量 | 18.9 kDa |
| 表观分子量 | 16 kDa |
| 生物活性 | Measured in a cell proliferation assay using FDC-P1 Mouse bone marrow cells. The ED50 for this effect is 6 ng/mL. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2~8℃ for 2~7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
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文献和实验Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
strains. 1. Use C57BL/6 F1 mice to set up new breeding cages (see mouse breeding suggestions above). Most C57BL/6 F1 hybrids have good reproductive performance. We prefer (C57BL/6J X DBA/2J)F1 animals. Outbred CD-1 and ICR mice can be used
Transgenic Mouse Core Facility
precipitate Add 1/10th volume 3M NaOAc, pH=6.0 to aqueous layer. Add 2X volume EtOH. -20oC for at least 15 min. Microfuge at 4oC X 15 min. Remove EtOH. Air Dry. Resuspend each pellet in 50ul 10mM Tris, pH=7.5, 0.1mM EDTA. Combine into 1 tube
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