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huayun / IL-1RL1/ IL-1 R4 (C-6

His), Mouse, Recombinant,HYPC5239
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  • ¥3840
  • 华韵生物(Huayunbio)
  • HYPC5239
  • 2025年07月13日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      广州华韵生物科技有限公司

    • 规格

      10µg

    产品信息

    别名Interleukin-1 Receptor-like 1; Lymphocyte antigen 84; Protein ST2; Protein T1; Il1rl1; DER4; Fit-1; IL-1 R4; IL-1RL1; IL-33R; Ly84; ST2L; ST2V; suppression of tumorigenicity 2
    物种Mouse
    表达宿主Human Cells
    序列信息Ser27-Ala337
    检索号P14719-2
    分子量36.6 kDa
    表观分子量55-70 kDa
    标签C-6His
    生物活性 Immobilized Mouse ST2-His at 5μg/ml (100 μl/well) can bind Rhesus Macaque IL-33-His.The ED50 of Rhesus Macaque IL-33-His is 82.03 ng/ml.

    产品特性

    纯度 >95% as determined by reducing SDS-PAGE.
    内毒素< 1.0 EU per µg as determined by LAL test.
    保存 Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2~8℃ for 2~7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months.
    运输 Ambient temperature or ice pack.
    制剂Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
    复融Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    背景介绍

    ST2, also called IL-1 R4, is an Interleukin-1 Receptor family glyco Protein that plays a role in Th2 immune responses. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. This Receptor is very similar to the IL-1 Receptor type I and the IL-18 Receptor α chain in that ST2 also has three extracellular Ig domains and an intracellular Toll domain. ST2 binds IL-33, enhances inflammatory Cytokines by activating nuclear factor-κB (NF-κB) and mitogen activated Protein (MAP) kinases. ST2 exists as either a membrane bound form (ST2L) or as a soluble form (sST2). ST2L acts as a transmembrane signalling Receptor for IL-33 by mediating the effect of IL-33 on the inflammatory process, while sST2 can suppress IL-33 activity.

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    图标文献和实验
    相关实验
    • Immunohistochemistry: Fluorescence Protocol 2.0

      . Sample Preparation and Fixation Harvest cells and wash twice in V-bottomed tubes with cold Wash Buffer by centrifugation (400 x g for 5 minutes) at 4°C to remove extracellular cytokines. Add 0.5 mL of the Fixation Buffer

    • Immunohistochemisty-Fluorescence Protocol

      the cells twice by centrifugation in Wash Buffer-Saponin (400 x g for 5 minutes). Incubate cells for 30 minutes at room temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1, IgG2A or IgG2B diluted

    • Cytokine Bioassays

      virus solution at a density of 1-4 x 104 pfu/ml (see Quick Guide Chart for human IFN-gamma: 1/250 dilution of stock of 107 pfu/ml, for mouse IFN-gamma, 1/1000 dilution of stock of 107 pfu/ml). Incubate plate for 40 hours at 37°C, 5% CO2

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