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huayun / CD253/ TNFSF10/ TRAIL

, Human, Recombinant,HYPC5220
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  • ¥2340
  • 华韵生物(Huayunbio)
  • HYPC5220
  • 2025年07月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      广州华韵生物科技有限公司

    • 规格

      10µg

    产品信息

    别名Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand; Apo-2L; TNF-Related Apoptosis-Inducing Ligand; Protein TRAIL; CD253; TNFSF10; APO2L; TRAIL
    物种Human
    表达宿主E.coli
    序列信息Arg115-Gly281
    检索号P50591
    分子量19.5 kDa
    表观分子量16 kDa
    生物活性 Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 1-10 ng/ml.

    产品特性

    纯度 >95% as determined by reducing SDS-PAGE.
    内毒素< 1.0 EU per µg as determined by LAL test.
    保存 Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
    运输 Ambient temperature or ice pack.
    制剂Supplied as a 0.2 μm filtered solution of 40mM Tris-HCl, 300mM NaCl, 5%Trehalose, 5%Mannitol, 0.01%Tween80, 10%Glycerol, pH7.0.

    背景介绍

    Human TNFSF10 is a type II transmembrane Protein with an intracellular N-terminus and a ‘TNF homology domain’ (THD) at the extracellular C terminus. TNFSF10 can interact with several distinct Receptors. Two of these Receptors that belongs to TNFR superfamily, DR4 (TRAIL-R1) and DR5 (TRAIL-R2/ TRICK2), are plasma membrane Proteins containing intracellular death domains essential for activating apoptosis. TNFSF10 is promising for cancer therapy because it is cytotoxic and activates apoptosis in the majority of malignant cells, but not in normal cells.

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    图标文献和实验
    相关实验
    • Activation and Expansion of Human Treg Cells

      to TCR/CD3 and CD28 for full activation and expansion of human Treg cells. 二、实验试剂 2 ml Dynabeads? Human Treg Expander 2 x 107 beads/ml in phosphate buffered saline (PBS), pH 7.4, w/ 0.1% human serum albumin (HSA). Magnet: Any Dynal MPC Buffer

    • 【分享】Human press METHODS IN MOLECULAR BIOLOGY

      9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols

    • 染色质 免疫沉淀 反应

      -ChIP and X-ChIP. - http://www.abcam.com/index.html?pageconfig=resource&rid=10418&CFID=2422773&CFTOKEN=36329956 Expert protocols and tips for N-ChIP and X-ChIP. AbCAM Immunoprecipitation of Chromatin from Fixed Yeast Protocol PDF - http

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