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- HYPC5219
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州华韵生物科技有限公司
- 规格：
10µg
产品信息
| 别名 | Tumor necrosis factor Receptor superfamily member 1B; TNFRSF1B; Tumor necrosis factorReceptor 2; TNF-R2; TNF-RII; Tumor necrosis factor Receptor type II; p75; p80 TNF-alphaReceptor; CD120b |
| 物种 | Human |
| 表达宿主 | Human Cells |
| 序列信息 | Pro24-Thr206 |
| 检索号 | P20333 |
| 分子量 | 46.5 kDa |
| 表观分子量 | 60 kDa |
| 生物活性 | Immobilized Recombinant Human TNF RII-mFc at 1μg/ml (100 μl/well) can bind Recombinant Human TNF alpha-His. The ED50 of Recombinant Human TNF alpha-His is 1.97 ng/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2~8℃ for 2~7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4. |
| 复融 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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文献和实验Activation and Expansion of Human Treg Cells
to TCR/CD3 and CD28 for full activation and expansion of human Treg cells. 二、实验试剂 2 ml Dynabeads? Human Treg Expander 2 x 107 beads/ml in phosphate buffered saline (PBS), pH 7.4, w/ 0.1% human serum albumin (HSA). Magnet: Any Dynal MPC Buffer
/farnham/protocols/tissues.html Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues. Farnham Lab. Chromatin Immunoprecipitation Protocol - /attach/2008/2008-02-01/FF/FF194C6609B9F73761BD4D317C7D1BCC.pdf ChIP Staph A cell preparation
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
. 36. Resuspend the cells in OP9 medium, seed 5 x 105 cells per 10-cm dish containing ~80% confluent OP9 cells in 10 mL of OP9 medium, and add 5 ng/mL of recombinant human Flt-3L. Return the dish to the incubator. The number of 10-cm dishes
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