- ￥2640
- 华韵生物(Huayunbio)
- HYPC5069
- 2025年07月15日
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- 详细信息
- 技术资料
- 供应商：
广州华韵生物科技有限公司
- 规格：
10µg
产品信息
| 别名 | Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2 |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | Val77-Leu233 |
| 检索号 | P01375 |
| 分子量 | 17.5 kDa |
| 表观分子量 | 16 kDa |
| 生物活性 | Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | <1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2~8℃ for 2~7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 6.0. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
TNFα is a homotrimer with a subunit molecular mass of 17 kD Cytokine that binds to TNFRSF1A/ TNFR1 and TNFRSF1B/ TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease.
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huayun / TNF-α/ TNFA/ TNFSF2, Human, Recombinant，HYPC5069
￥2640
