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- Invitrogen™
- W11261
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
需确认
- 英文名：
Wheat Germ Agglutinin (WGA)
- CAS号：
见产品外包装
- 保质期：
见产品外包装
- 供应商：
嵘崴达
- 保存条件：
见产品外包装
- 规格：
5 mg
Wheat Germ Agglutinin (WGA)
货号 W11261
产品描述
Thermo Fisher Scientific offers a broad selection of fluorescent wheat germ agglutinin conjugates. These lectins can bind to carbohydrates and are available conjugated to Alexa Fluor™, Alexa Fluor™ Plus, and other fluorescent dyes. Fluorescent wheat germ agglutinin conjugates are valuable tools in molecular and cell biology research, enabling researchers to label the plasma membrane in fluorescence imaging and cell painting assays, and study and analyze glycosylation patterns and glycan-mediated processes in cells and tissues.
Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) with Alexa Fluor, Alexa Fluor Plus, and other dyes. WGA is a cell impermeant stain that selectively binds to N‐acetylglucosamine and N‐acetylneuraminic acid (sialic acid) residues, which are often found on cell membranes. Fluorescent WGA conjugates provide selective labeling of the plasma membrane with minimal background in many cell types that is retained after formaldehyde fixation and permeabilization with Triton X-100.
These fluorescent lectin conjugates can also be used to label fixed cells; however, to avoid labeling intracellular components, formaldehyde-fixed cells should not be permeabilized before labeling. Fluorescent WGA conjugates are used as plasma membrane stains along with other cellular markers in cell painting assays to provide a phenotypic readout of cell health or cytotoxicity. The Wheat Germ Agglutinin, Alexa Fluor 555 Conjugate is included in the Image-iT Cell Painting Kit (Cat. Nos. I65000 and I65500).
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文献和实验Translation Using a Wheat-Germ Extract
Wheat-germ extract provides a reliable, convenient, and easy-to-use system to initiate translation from a wide variety of viral, prokaryotic, and eukaryotic mRNAs and produce full-length polypeptide products (1 ). Translation reactions in vitro
Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
of properly folded, eukaryotic proteins remains a challenging task. Current wheat-germ-based cell-free in vitro translation systems present a plausible alternative for protein synthesis since majority of eukaryotic proteins could be obtained in their native
In Vitro Translation of mRNA in a Wheat Germ Extract Cell-Free System
The wheat germ extract in vitro translation system has been used widely for faithful and efficient translation of viral and eukaryotic messenger RNAs in a heterologous cell-free system (1 –9 ). With respect to the yield of translation products
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