Anti-Myosin light chain kinase

Purification of Myosin Light Chain Kinase

RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant

Generation of Antibody Molecules Through Antibody Engineering

domains of the heavy (V H ) and light (V L ) chains. Effector functions are determined by constant C H 2 and C H 3 domains. The picture is based on the crystal structure of an intact IgG2 anti-canine lymphoma MAb231 ( 2 ) (pdb entry 1IGT). The drawing

Selection of Human Immunoglobulin Light Chains from a Phage-Display Library

for the substrate ground state and a more flexible active site than the intact antibody-combining site. Second, Fv species from combinatorial libraries of heavy (H)- and L-chain-variable regions are not likely to reflect the composition of antibodies found in vivo