Biotin Anti-MRP8 + MRP14 antib

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands

Introduction of human 14-3-3 proteins

of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins.A polyclonal antibody was raised against purified ST-IV.A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti

ELISA Procedure for Measuring Serum Antibody Titer

of an antibody is shown in Figure 22. The titer was estimated to be 1 in 2400. Figure 22. Antibody titer graph (X = dilution corresponding to Ab titer) Further Methods for Evaluating Anti-peptide Antibodies If the antibodies are to be used as a reagent