Anti-COX1 / Cyclooxygenase 1 a

Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

7.16 anti-rat IgG2b). -------------------------------------------------------------------------------- 5) For full details see: Clark, M.R., & Waldmann, H. Production of murine monoclonal antibodies in Methods in Hematology 13:1-20 (1986

E L I S A w i t h p l a t e l e t s

for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS

双荧光素酶检测的原理和应用

过程相对简单，检测灵敏度较高；Western blotting操作过程繁琐，检测灵敏度较低。3、荧光素酶报告基因检测只需要成功将含有目标DNA序列插入到报告载体中，即可进行后续实验，Western blotting则需要购买商品化的一抗，一般价高量少，且抗体的杂交条件需要摸索；若自制的一抗，抗体效价需要验证，实验周期较长。六、参考文献Pan, Y., R. Geng, N. Zhou, G. F. Zheng, H. Zhao, J. Wang, C. M. Zhao, X. B. Qiu, Y. Q