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文献和实验Secondary structure of prion mRNA
, including scrapie in sheep, bovine spongiform encephalopathy (BSE) in cattle, and Creutzfeldt-Jakob disease (CJD) in man. During prion infection, an abnormal isoform of PrP, in the case of scrapie designated PrP Sc , is produced from the cellular isoform
In situ hybridization of yeast cells (RNA and Oligonucleotide probes)
protocol is adapted from Long RM, et al. RNA 1995 Dec;1(10) :1787-1794 . II. Fixation: Yeast are grown in 45ml cultures in the appropriate media until they reach early log phase (OD600 between 0.2 and 0.4). Cells are fixed for 10
In situ hybridization of yeast cells (RNA and Oligonucleotide probes)
is adapted from Long RM, et al. RNA 1995 Dec;1(10) :1787-1794. II. Fixation: Yeast are grown in 45ml cultures in the appropriate media until they reach early log phase (OD600 between 0.2 and 0.4). Cells are fixed for 10 minutes at room
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