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文献和实验Characterization of Histaminergic Receptors
Additional reagents and equipment for preparing guinea pig cortical membranes ( protocol 2 ) Basic Protocol 3: Measurement of [3H]N‐α‐Methyl Histamine Binding to Cloned Human H3 Receptors in Membranes Materials Cell
should cover it. Incubate at RT for 1 hr or longer, depending on the antibody. Sometimes an overnight inc ubation is helpful. Jon's anti-actin Guinea pig antibodies from animal#1 are best at 1:6,000, #2 and #3 are best at 1:2,000. Wash the cells 4 x
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
although other techniques can be used. The choice of immobilized antibody binding protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b . Protein G binds
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