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文献和实验Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.3 - Extraction of Chromatin LEVEL II Materials Bovine or porcine brain 0.25 M Sucrose containing 0.0033 M calcium
Southern Blot Hybridization of Digoxigenin-Labeled RNA Minisatellite Probes and Color Detection
fingerprints in three different species using digoxigenin-labeled RNA minisatellite 33.15 and color detection: ( a ) in man, ( b ) in the common marmoset ( Callithrix jacchus ), and ( c ) in the Waldrapp ibis ( Geronticus eremita ). F, father; M, mother; Dl
Tissue sectioning: Cryostat sectioning method
% sucrose in PBS overnight or in 7.5% porcine gelatin (300 Bloom) 15% sucrose in PBS for 6 h at 37oC. * Embed in 15% sucrose/7.5% gelatin, chill to 4oC (you can hold the samples here for a few days). * Remove sucrose/gelatin solution and add Tissue
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