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文献和实验Observation of living and plastic-embedded chick embryos
is that the embryo is relatively accessible and can be operated on or treated with teratogens (substances that perturb development) while still in ovo(in the egg). Finally, fertilized chicken eggs are available year round and can be "held" at 10-14ºC
How to Extract DNA from Anything Living
contains DNA. For this experiment, we like to use green split peas. But there are lots of other DNA sources too, such as: Spinach Chicken liver Onions Broccoli Here's the fun part. Put in a blender:
Gene Knockout In Murine Embryonic Stem (ES) Cells
from the PMEFs as this is a differentiating agent). Also check ES cells, at this point they should be a nice dense monolayer, try to get a feel for the numbers, don't want too many cells (with time doing this it should be possible to have an accurate number
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