Anti-Creatine Kinase MM antibo

类器官培养与应用——肾类器官

R K, et al. Generation of patterned kidney organoids that recapitulate the adult kidney collecting duct system from expandable ureteric bud progenitors[J]. Nature Communications, 2021, 12(1): 3641. 【2】Nishita M, Qiao S, Miyamoto M, et al. Role of Wnt

Anti-P-CREB

to nitrocellulose membrane. I include in the SDS-PAGE prestained molecular weight markers to check the efficiency of the transfer and for M.W. determination.7. After transfer, rinse the blot 2 times with TBST (10 mM Tris (pH 7.4), 150 mM NaCl, 0.05% Tween-20

Anti-HA Immunocytology

and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC