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文献和实验GST-fusion protein injection into rabbits
. Combine eluted fractions (El1+El2) in 50 ml falcon tube, freeze at -80oC, close with puncture plastic cap. Lyophilize over-night. Resuspend in 0.5-1 ml of DDW, spin, and transfer to a small tube. Store at -20oC. Rabbit Screening It is important
Making antibodies to synthetic pepti
at once. We shave the upper back of the rabbit prior to immunization. We tend to use 1 mg of peptide per immunization (3-5 intradermal sites at each immunization), and re-immunize at 2-6 week intervals. Intradermal (intracutaneous) means: raise a wheal (inject
Making antibodies to synthetic peptides
at once. We shave the upper back of the rabbit prior to immunization. We tend to use 1 mg of peptide per immunization (3-5 intradermal sites at each immunization), and re-immunize at 2-6 week intervals. Intradermal (intracutaneous) means: raise a wheal (inject
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