- ￥5557.50
- 华韵生物(Huayunbio)
- HFC3160-100Tests
- 2025年07月13日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.
|
| Alternate Names |
CD49 antigen-like family member D;CD49d;ITGA4;Integrin alpha-4;Integrin alpha-IV;VLA-4 subunit alpha
|
| Swissprot |
P13612
|
| Gene ID |
3676
|
| Clone No |
9F10
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
Elab Fluor® 488 Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
Elab Fluor488
|
| Conjugation Information |
Elab Fluor 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
This protocol is for use with the D and E cyclins and employs 488 nm argon laser excitation of propidium iodide and a FITC-conjugated phenotypic label, and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression. It has been tested with antibodies
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