- ￥1638
- 华韵生物(Huayunbio)
- HFC2475-100μg
- 2025年07月12日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.
|
| Alternate Names |
CD90.2;Thy-1.2;Thy-1.2 antigen;Thy-1.2 membrane glycoprotein;Thy1.2
|
| Gene ID |
21838
|
| Clone No |
30H12
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
FITC Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Dual- and Triple-Color Fluorospot
functional antibody pairs are derived from a few species. Another strategy being employed for triple staining is the use of additional tag/anti-tag systems other than FITC/anti-FITC (Fig. 1). 实验试剂 1. Blocking reagent after coating: 10
细胞类别MACS分选产品流式细胞仪抗体干祖细胞Anti-Sca-1 MicroBeadsAnti-Sca-1 Multisort KitCD117 MicroBeadsLineage Cell Depletion Kit T细胞及T细胞亚群CD90(Thy1.2) MicroBeadsPan T Cell Isolation KitCD4(L3T4) MicroBeadsCD4+ T Cell Isolation Kit CD4+CD25+ Regulatory T Cell
Stewart Laboratory Standard Operating Procedure
alone stain is desired, set aside a fraction of cells until step 10). Resuspend cell pellet in anti-BrdU antibody (50 μL total solution diluted with wash buffer). Incubate for 20 minutes at 4°C. Use BrdU-FITC: Boeringher #1202693 1:100 dilution (skip to step
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