- ￥2340
- 华韵生物(Huayunbio)
- HFC2255-100μg
- 2025年07月13日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as FcγRII and FCRII. It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells. There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing. CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).
|
| Alternate Names |
IGFR2;CD32;CDw32;FCG2;FCGR2B;Fc-gamma RII-b;Fc-gamma-RIIb;FcRII-b;IgG Fc receptor II-b;Low affinity immunoglobulin gamma Fc region receptor II-b
|
| Swissprot |
P31994
|
| Gene ID |
2212
|
| Clone No |
IV-3
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2b, κ
|
| Isotype Control |
Biotin Mouse IgG2b, κ Isotype Control[MPC-11]
|
| Conjugation |
Biotin
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Dual- and Triple-Color Fluorospot
stimulatory agents of choice and anti-CD28 (0.1 μg/ml; seeNote 4), in a final volume of 100–150 μl/well. The number of cells per well is dependent on the stimulating agent used (seeNote 5). b. Incubate the plate overnight at 37°C in a humidified
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
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