- ￥1404
- 华韵生物(Huayunbio)
- HFC2079-100μg
- 2025年07月13日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD21 is a 145 kD transmembrane protein also known as complement C3d receptor (C3dR), complement receptor 2 (CR2), and Epstein-Barr virus receptor. CD21 is expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, and some epithelial cells. CD21 is the receptor used by Epstein-Barr virus to infect B cells and is also the complement receptor for C3d. CD21 has also been shown to interact with a number of proteins, including CD23, CD19, annexin VI, CD81, iC3b, complement receptor 1 (CR1, CD35), and interferon-alpha 1 (IFN-α1).
|
| Alternate Names |
CD21;CR2;Complement C3d receptor;Complement receptor type 2;Cr2;EBV receptor;Epstein-Barr virus receptor
|
| Swissprot |
P20023
|
| Gene ID |
1380
|
| Clone No |
BU32
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
Purified Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
; the more the better) with 0.25-0.5 μg anti-PAK antibody per 100 μg of lysate for 1.5hrs at 4oC with gentle rocking. 6. Add 25 μl protein A-, protein G-, or protein A/G-beads and incubate for 30min at 4oC with gentle rocking. 7. During this incubation, make
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
Assays (See Note 9) 1. Pre-incubate anti-GSK3β (5 μg) monoclonal antibody withprotein G-Sepharose (400 μL of a 50% slurry) in kinasebuffer at 4°C for 1 h to conjugate the antibody to the proteinG-Sepharose beads. 2. Centrifuge the beads at 6,000× g
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