- ￥2340
- 华韵生物(Huayunbio)
- HFC2070-100Tests
- 2025年07月16日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
|
| Alternate Names |
B-lymphocyte antigen CD20;B-lymphocyte surface antigen B1;Bp35;CD20;Leukocyte surface antigen Leu-16;MS4A1;Membrane-spanning 4-domains subfamily A member 1
|
| Swissprot |
P11836
|
| Gene ID |
931
|
| Clone No |
BCA/B20
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
PE Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验避光染色30分钟至过夜。 最后用相同破膜缓冲液洗涤两次，重悬后通过流式细胞仪采集数据。该方案兼顾报告荧光稳定性与核/胞质蛋白染色效率，适用于多参数精细免疫表型分析。 菲恩生物提供专业流式配色设计与高质检测试剂，优化多色方案，降低干扰，提升检测精准度，助力免疫细胞精细分选，赋能免疫学、肿瘤免疫及自身免疫疾病研究。欢迎合作，共促创新！ 以下是菲恩生物推荐的流式抗体搭配方案 种属 细胞群 流式抗体搭配 货号 Human T/B/NK细胞群检测 CD
-Human CD20 mAb,PE 50T/100T 1000/1500 L0062 Mouse anti-Human CD20 mAb,PE-Cy5 50T/100T 1200/1800 L0063 Mouse anti-Human CD20 mAb,APC 50T/100
血单个核细胞（PBMC）。样本采集后24小时内检测。 试剂： 1. 检测DC细胞用的BDIS荧光标记的单克隆抗体。 四色分析： LIN1 FITC：货号340546，含有CD3、CD14、CD16、CD19、CD20、CD56 CD123 PE（Anti-IL-3Ra）：货号340545 Anti-HLA-DR PerCP：货号347364 CD11c APC：货号340544 Mouse IgG1 PE：货号349043 Mouse IgG2a APC：货号340473
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