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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100μg
请联系华韵客服获取说明书。
产品简介:
| Background |
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
|
| Alternate Names |
CD3;CD3E/D/G/Z;CD3e/d/g/z;T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain
|
| Swissprot |
P04235;P11942;P22646;P24161;
|
| Gene ID |
12502
|
| Clone No |
17A2
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
Biotin Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
Biotin
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
Dual- and Triple-Color Fluorospot
. Spontaneous production is determined by incubating the same number of cells in the absence of antigen. A polyclonal activator, such as anti-CD3 mAb (100 ng/ml) or phytohemagglutinin (PHA; 1–10 μg/ml), should normally be used as a control for cell viability
107 的 PBMC 细胞用 100μl 的 Buffer 稀释(Buffer CI:含 0.5% BSA 的 PBS 缓冲液,1 mM EDTA) · 加入多种生物素化抗体,室温下孵育 5 分钟。生物素化的抗体包含以下几种,每种抗体 0.5 μg:CD235a (erythrocytes), CD14 (monocytes), CD3 (T cells), CD15 (granulocytes), CD19 (B cells), CD36 (thrombocytes), CD123
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