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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100μg
请联系华韵客服获取说明书。
产品简介:
| Background |
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
|
| Alternate Names |
CD3;CD3E/D/G/Z;CD3e/d/g/z;T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain
|
| Swissprot |
P04235;P11942;P22646;P24161;
|
| Gene ID |
12502
|
| Clone No |
17A2
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
Purified Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法: 货号 名称
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
volume of Dynabeads taken from the vial (step 2). 2. Activation of Mouse T Cells 1) Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2) Add 2 µl Dynabeads Mouse T-Activator CD3/CD
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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