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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100μg
请联系华韵客服获取说明书。
产品简介:
| Background |
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.
|
| Alternate Names |
CD16a/b;CD32;CD32A/B;FCG2A;FCGR2A/BFCGR3;FCGR3A/B;Fc fragment of IgG low affinity IIIa/b receptor;Fc fragment of IgG low affinity IIIb receptor;Fc fragment of IgG low affinity IIa/b receptor;Fc gamma RIIa/b ;Fc gamma receptor III A/B;FcGR
|
| Swissprot |
P08508;P08101;
|
| Gene ID |
14130,14131
|
| Clone No |
2.4G2
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
Elab Fluor® 647 Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
Elab Fluor647
|
| Conjugation Information |
Elab Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验为解决转录因子(TF)染色过程中报告荧光蛋白信号丢失的问题,研究人员首先在CD4-Cre × EYFP小鼠脾细胞中比较了不同固定/破膜方案。进一步实验显示,Fix/Perm处理后的上清液中蛋白含量显著高于Triton X-100处理组,且通过抗GFP抗体捕获法可在Perm处理后的上清中检测到EYFP,但在先经Fix/Perm处理的样本中则无法检出——说明EYFP主要在Fix/Perm步骤中因固定不充分而泄漏。这些结果共同证实:荧光丢失源于固定强度不足,而非荧光团降解。 尽管2%多聚甲醛(PFA
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
')2 Fragment(Alexa Fluor® 555 Conjugate)#4413 Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 647 Conjugate)#4414 抗小鼠: Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4408 Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor
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