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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100μg
请联系华韵客服获取说明书。
产品简介:
| Background |
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
|
| Alternate Names |
Gpf480;Adgre1;Adhesion G protein-coupled receptor E1;Cell surface glycoprotein F4/80;EGF-like module receptor 1;Emr1
|
| Swissprot |
Q61549
|
| Gene ID |
13733
|
| Clone No | |
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
Elab Fluor® Violet 450 Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
Elab FluorViolet 450
|
| Conjugation Information |
Elab Fluor Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Studies of the Ubiquitin Proteasome System
phosphatase inhibitors and 50 µM MG132 (or lactacystin) Anti‐E3 or anti‐substrate antibody Nondenaturing Triton X‐100 wash buffer (see recipe ) Tris‐buffered saline (TBS
Cruz, CA), anti-CD49f (clone GoH3, PharMingen) and secondary antibodies conjugated to phycoerythrin (PE, 1:100 dilution, Jackson ImmunoResearch, West Grove, PA). Next, 5 105 to 106 GFP+ PE+ infected cells (3ZF library) or 107 GFP+ PE+ infected cells
for detection of the FISH signals BSA (Sigma, 98% electrophoresis purity) Normal donkey or goat serum (Jackson laboratories) Blocking solution: PBS 1X; 0.3% Triton X-100; 1% BSA; 10% normal donkey or goat serum FITC-anti-Biotin
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