- ￥1404
- 华韵生物(Huayunbio)
- HFC1608-100μg
- 2025年07月14日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
|
| Alternate Names |
Activation B7-1 antigen;B7;Cd80;T-lymphocyte activation antigen CD80
|
| Swissprot |
Q00609
|
| Gene ID |
12519
|
| Clone No |
16-10A1
|
| Host |
Armenian Hamster
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Armenian Hamster IgG
|
| Isotype Control |
Biotin Armenian Hamster IgG Isotype Control[PIP]
|
| Conjugation |
Biotin
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
注：此后步骤应注意避光操作。 1.2 滴加一抗 将4 μl Anti-DIG monoclonal antibody 和0.5 μl Texas-red-Avidin 稀释于100 μl 抗体稀释液1中，混匀；每张玻片滴加25 μl 于杂交区域，盖上22mm×22mm盖玻片，于37℃湿盒中孵育1小时；室温下4×SSC/0.1 % Tween-20中振荡洗涤5min×2缸。 1.3 滴加二抗
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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