- ￥1638
- 华韵生物(Huayunbio)
- HFC1547-100μg
- 2025年07月13日
- FCM
企业认证
相关产品推荐更多 >
Anti-FKBP12 antibody ，应用: WB,IHC,IF/ICC，反应: H, M, R，HY- ab92459 -50ul
￥1800Anti-Ferritin antibody ，应用: WB,IHC,IF/ICC，反应: H, M, R，HY- ab76768 -50ul
￥1800
Anti-NRDP1 antibody ，应用: WB,IHC,IF/ICC，反应: H, M, R，HY- ab235336 -50ul
￥1800
Anti-HINT1 antibody ，应用: WB,IHC,IF/ICC，反应: H, M, R，HY- ab124912 -50ul
￥1800
Anti-Cytokeratin 19 antibody ，应用: WB,IHC,IF/ICC，反应: H, M, R，HY- ab255939 -50ul
￥1800
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.
|
| Alternate Names |
CD161 antigen-like family member C;CD161/NK1.1;CD161c;Killer cell lectin-like receptor subfamily B member 1C;Klrb1c;Ly-55c;NKR-P1 40;NKR-P1.9;NKR-P1C
|
| Swissprot |
P27814;P27812;Q99JB4;
|
| Gene ID |
17059
|
| Clone No |
PK136
|
| Host |
Mouse
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
PE Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Intracellular Cytokine Staining Protocol
28 (2ug/ml soluble) 5hrMonensinJES5-16E3IL-12mouse PECmIFNg (100ng/ml) (2hr)/LPS (100ng/ml) (22hr)2hr/22hr-MonensinC17.8GM-CSFmouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble
Intracellular Cytokine Staining Protocol
(100ng/ml)(22hr) 2hr/22hr - Monensin B122 IL-2 mouse spleen ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized
为解决转录因子（TF）染色过程中报告荧光蛋白信号丢失的问题，研究人员首先在CD4-Cre × EYFP小鼠脾细胞中比较了不同固定/破膜方案。进一步实验显示，Fix/Perm处理后的上清液中蛋白含量显著高于Triton X-100处理组，且通过抗GFP抗体捕获法可在Perm处理后的上清中检测到EYFP，但在先经Fix/Perm处理的样本中则无法检出——说明EYFP主要在Fix/Perm步骤中因固定不充分而泄漏。这些结果共同证实：荧光丢失源于固定强度不足，而非荧光团降解。 尽管2%多聚甲醛（PFA
技术资料暂无技术资料 索取技术资料
