- ￥1677
- 华韵生物(Huayunbio)
- HFC1522-100μg
- 2025年07月15日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
|
| Alternate Names |
B-lymphocyte antigen CD19;CD19;Cd19;Differentiation antigen CD19
|
| Swissprot |
P25918
|
| Gene ID |
12478
|
| Clone No |
1D3
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
PE Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验为解决转录因子（TF）染色过程中报告荧光蛋白信号丢失的问题，研究人员首先在CD4-Cre × EYFP小鼠脾细胞中比较了不同固定/破膜方案。进一步实验显示，Fix/Perm处理后的上清液中蛋白含量显著高于Triton X-100处理组，且通过抗GFP抗体捕获法可在Perm处理后的上清中检测到EYFP，但在先经Fix/Perm处理的样本中则无法检出——说明EYFP主要在Fix/Perm步骤中因固定不充分而泄漏。这些结果共同证实：荧光丢失源于固定强度不足，而非荧光团降解。 尽管2%多聚甲醛（PFA
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Mouse anti-Human CD19 mAb,FITC 50T/100T 800/1200 L0055 Mouse anti-Human CD19 mAb,PE 50.100T 1000/1500 L0056 Mouse anti-Human CD19 mAb,PE-Cy5
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