- ￥3393
- 华韵生物(Huayunbio)
- HFC3988-100Tests
- 2025年07月15日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
|
| Alternate Names |
CD62L;CD62 antigen-like family member L;L-selectin;LAM-1;LECAM1;Lnhr;Ly-22;Ly22;Lymph node homing receptor;Sell
|
| Swissprot |
P14151
|
| Gene ID |
6402
|
| Clone No |
HI62L
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
PE Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验表面标志物，比如 CD62L，对较高的温度条件往往比较敏感。部分 MHC 四聚体已被证实，在 37℃ 条件下容易与细胞解离。故我们建议：在 4℃ 条件下孵育 45-60 分钟，或在室温条件下孵育 30 分钟。3.在进行大规模试验前，建议先进行预试验，以确定最佳的 MHC 四聚体稀释比例。通常，建议 MHC 四聚体的初始稀释比例为 1:100～1:200。请根据具体实验，优化其稀释比例，以期获得最佳的染色效果。4.对于新鲜的外周血单核细胞、淋巴细胞、脾细胞，通常建议每个染色样品细胞总数为 1-2 ×106；而对于细胞
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
PE‐labeled anti‐human HLA‐A,B,C antibody (4 µl/200 µl cells; BD Biosciences, cat. no. 555553) Mouse IgG1 FITC antibody (4 µl/200 µl cells; BD Biosciences, cat. no. 555748
; stippled line, control staining without primary antibody. Screening for upregulation of target genes in human cell lines. We delivered millions of TFZF s into the human squamous carcinoma cell line A431 using a retroviral vector, pMX-IRES-GFP15 (Fig
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