- ￥2340
- 华韵生物(Huayunbio)
- HFC3916-100Tests
- 2025年07月16日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-.
|
| Alternate Names |
Hematopoietic progenitor cell antigen CD34
|
| Swissprot |
Q64314
|
| Gene ID |
12490
|
| Clone No |
RAM34
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
PE Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
) anti-hCD14-PE anti-hCD19-PE anti-hCD34-PE anti-hCD45-CyChrome Use corresponding PE-conjugated mouse IgG controls to evaluate specific labeling (see Step 19). CalPhos Mammalian
Mouse Th1/Th2 细胞群检测 CD3-PerCP-Cy5.5 PCP55-30002 CD4-FITC FITC-30128 IFN-γ-PE PE-30074 IL4-APC APC-30026 Human Treg细胞群检测 CD4-FITC FITC-30005 CD25-PE PE-30035 CD3-PerCP-Cy5.5 PCP55-30004 CD127-FineTest®647
Mouse anti-Human CD34 mAb,FITC 50T/100T 800/1200 L0086 Mouse anti-Human CD34 mAb,PE 50T/100T 1000/1500 L0087 Mouse anti-Human CD34 mAb,Biotin
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