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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100Tests
请联系华韵客服获取说明书。
产品简介:
| Background |
CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.
|
| Alternate Names |
Activation B7-2 antigen;B7-2;B7.2;B70;BU63;CD28LG;CD28LG2;CD86;CTLA-4 Counter-Receptor B;CTLA-4 Counter-Receptor B7.2;ETC;ETC-1;Early T-cell costimulatory molecule;Early T-cell costimulatory molecule 1;FUN;FUN-1;LAB;LAB72;T-lymphocyte activation antigen C
|
| Swissprot |
P42081
|
| Gene ID |
942
|
| Clone No |
IT2.2
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2b, κ
|
| Isotype Control |
FITC Mouse IgG2b, κ Isotype Control[MPC-11]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验避光染色30分钟至过夜。 最后用相同破膜缓冲液洗涤两次,重悬后通过流式细胞仪采集数据。该方案兼顾报告荧光稳定性与核/胞质蛋白染色效率,适用于多参数精细免疫表型分析。 菲恩生物提供专业流式配色设计与高质检测试剂,优化多色方案,降低干扰,提升检测精准度,助力免疫细胞精细分选,赋能免疫学、肿瘤免疫及自身免疫疾病研究。欢迎合作,共促创新! 以下是菲恩生物推荐的流式抗体搭配方案 种属 细胞群 流式抗体搭配 货号 Human T/B/NK细胞群检测 CD
Mouse anti-Human CD81 mAb,FITC 50T/100T 800/1200 L0150 Mouse anti-Human CD81 mAb,PE 50T/100T 1000/1500 L0151 Mouse anti-Human CD86 mAb,FITC
29 (MEM 101A, purified IgG1) was used as reported previously [9–11]. FITC-labeled antibodies to CD80, CD86, CD29, and CD18 were obtained from Immunotech (Marseilles, France).2.2. AnimalsC57BL/6 male mice (6–8 weeks old, 17–21 g) were obtained from DAEHAN
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