- ￥5135
- 华韵生物(Huayunbio)
- HFC3632-500μg
- 2025年07月09日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
500μg
请联系华韵客服获取说明书。
产品简介：
| Background |
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.
|
| Alternate Names |
B-cell IgG differentiation factor;B-cell growth factor 1;BSF-1;IGG1 induction factor;IL-4;Interleukin-4
|
| Swissprot |
P07750
|
| Gene ID |
16189
|
| Clone No |
11B11
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
ICFCM;Neut;Stim
|
| Isotype |
Rat IgG1, κ
|
| Isotype Control |
AF/LE Purified Rat IgG1, κ Isotype Control[HRPN]
|
| Conjugation |
None (AF/LE)
|
| Storage Buffer |
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Dual- and Triple-Color Fluorospot
for the other cytokine (Fig. 1). As a second step in the detection, streptavidin conjugated to a red fluorophore (Cy3) and anti-FITC antibodies labeled with a green fluorophore, respectively, are used (10, 11). The resulting spots are subsequently analyzed using
分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
Intracellular Immunofluorescent Staining for Flow Cytometry
Brefeldin A BVD6-24G2 , 11B11 IL-5 NEW! mouse splenic CD4 ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hr
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