- ￥2827.50
- 华韵生物(Huayunbio)
- HFC3595-100Tests
- 2025年07月12日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
L-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.
|
| Alternate Names |
CSIF;Cytokine synthesis inhibitory factor;IL-10;Interleukin-10
|
| Swissprot |
P18893
|
| Gene ID |
16153
|
| Clone No |
JES5-16E3
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
ICFCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
APC Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
APC
|
| Conjugation Information |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells
molecules CD40, CD80, and CD86 (5 ,6 ), and production of cytokines such as interleukin-12 (IL-12) (7 -9 ), IL-6 (10 ), and interferon α (IFN-α) (10 ,11 ). Moreover, DC can process antigens in various forms by different pathways and induce high levels
Protocol for intracytoplasmic staining of cytokines for FACS analysis
; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide
Intracellular Cytokine Staining Protocol
28 (2ug/ml soluble) 5hrMonensinJES5-16E3IL-12mouse PECmIFNg (100ng/ml) (2hr)/LPS (100ng/ml) (22hr)2hr/22hr-MonensinC17.8GM-CSFmouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble
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