- ￥2691
- 华韵生物(Huayunbio)
- HFC3432-100μg
- 2025年07月10日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
|
| Alternate Names |
CD45;L-CA;Ly-5;Ptprc;T200
|
| Gene ID |
19264
|
| Clone No |
A20
|
| Host |
Mouse
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
PE Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
为解决转录因子（TF）染色过程中报告荧光蛋白信号丢失的问题，研究人员首先在CD4-Cre × EYFP小鼠脾细胞中比较了不同固定/破膜方案。进一步实验显示，Fix/Perm处理后的上清液中蛋白含量显著高于Triton X-100处理组，且通过抗GFP抗体捕获法可在Perm处理后的上清中检测到EYFP，但在先经Fix/Perm处理的样本中则无法检出——说明EYFP主要在Fix/Perm步骤中因固定不充分而泄漏。这些结果共同证实：荧光丢失源于固定强度不足，而非荧光团降解。 尽管2%多聚甲醛（PFA
LY5a (CD45.2)-Biotin (recognizes C57) 1:100 Pharmingen 553771 Ly5b (CD45.1)-PE (recognizes SJL) 1:100 Pharmingen 553776 Streptavidin-TriColor
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