- ￥2145
- 华韵生物(Huayunbio)
- HFC4030-20Tests
- 2025年07月11日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
20Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
CD194, also known as CCR4, is a CC chemokine receptor. It binds CCL17 and CCL22 and is expressed on a subset of T and B cells, basophils, monocytes, and NK cells. Human Th2 cells are characterized by the expression of CCR4 and CCR8, and these receptors are regulated differently during Th2 development. Human peripheral blood Tregs can be divided into two distinct populations based on the expression of CCR4. Freshly isolated Tregs express CCR4 and presumably represent memory-type Tregs, and CCR4- Tregs require CD3-mediated activation to acquire a regulatory activity. Depletion of CCR4+ T cells leads to Th1-type polarization of CD4+ T cells and augmentation of CD8+ T cell responses to tumor antigens. CCR4 and its ligands are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
|
| Alternate Names |
CC-CKR-4;CKR4;CMKBR4;ChemR13;HGCN:14099;K5-5;MGC88293
|
| Swissprot |
P51679
|
| Clone No |
L291H4
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
APC Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
APC
|
| Conjugation Information |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验避光染色30分钟至过夜。 最后用相同破膜缓冲液洗涤两次，重悬后通过流式细胞仪采集数据。该方案兼顾报告荧光稳定性与核/胞质蛋白染色效率，适用于多参数精细免疫表型分析。 菲恩生物提供专业流式配色设计与高质检测试剂，优化多色方案，降低干扰，提升检测精准度，助力免疫细胞精细分选，赋能免疫学、肿瘤免疫及自身免疫疾病研究。欢迎合作，共促创新！ 以下是菲恩生物推荐的流式抗体搭配方案 种属 细胞群 流式抗体搭配 货号 Human T/B/NK细胞群检测 CD
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
/farnham/protocols/tissues.html Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues. Farnham Lab. Chromatin Immunoprecipitation Protocol - /attach/2008/2008-02-01/FF/FF194C6609B9F73761BD4D317C7D1BCC.pdf ChIP Staph A cell preparation
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