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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
20Tests
请联系华韵客服获取说明书。
产品简介:
| Background |
CD16 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD16 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD16 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD16 appears to be expressed later in the immune response than CD86. CD16 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
|
| Alternate Names |
Fc gamma receptor;Fc gamma receptor 3;FcγRIII
|
| Swissprot |
P08637;O75015;
|
| Gene ID |
941
|
| Clone No |
3G8
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
PerCP Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
PerCP
|
| Conjugation Information |
PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验血单个核细胞(PBMC)。样本采集后24小时内检测。 试剂: 1. 检测DC细胞用的BDIS荧光标记的单克隆抗体。 四色分析: LIN1 FITC:货号340546,含有CD3、CD14、CD16、CD19、CD20、CD56 CD123 PE(Anti-IL-3Ra):货号340545 Anti-HLA-DR PerCP:货号347364 CD11c APC:货号340544 Mouse IgG1 PE:货号349043 Mouse IgG2a APC:货号340473
加入 1 μg/106 细胞 Anti-Mouse CD16/CD32 抗体封闭荧光抗体 Fc 受体引起的非特异性染色。震荡混匀,4℃ 孵育 30 min(或室温避光 15 min)。Wash Buffer 液洗涤,1 500 rpm,离心 5 min,弃上清。4. 用适量体积 Wash Buffer 液重悬每个样本,将各组细胞等细胞数混合后分装到单染管和不染抗体管,作为流式检测时调节电压。5. 单染管中加入相应表面染色抗体,同时在每个样本中加入 1 μL percp CD3/106细胞和 0.5 μ
和应用进展相当迅速。在世界卫生组织(WHO)和国际免疫学会联合会(IUIS)的组织下,自1982年至1993年已先后举行了五次有关人类白细胞分化抗原的国际协作组会议(International workshop on human leukocyte differentiation antigens),并应用以单克隆抗体鉴定为主的聚类分析法,将识别同一分化抗原的来自不同实验室的单克隆抗体归为一个分化群,简称CD(cluster of differentiation)。在许多场合下,抗体及其识别的相应
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