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- 2025年07月13日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
25μg
请联系华韵客服获取说明书。
产品简介：
| Background |
IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.
|
| Alternate Names |
B-cell differentiation factor I;IL-5;Interleukin-5;T-cell replacing factor;TRF
|
| Swissprot |
P05113;P04401;
|
| Gene ID |
3567,16191
|
| Clone No |
TRFK5
|
| Host |
Rat
|
| Reactivity |
Human;Mouse
|
| Application |
ICFCM;ELISA
|
| Isotype |
Rat IgG1, κ
|
| Isotype Control |
Purified Rat IgG1, κ Isotype Control[HRPN]
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
PBMCs activated with PMA/Ionomycin for 24 hrs. Reference Tables Table 1: Mouse ELISPOT Ready-Set-Go! Sets & Antibody Selection Quick Guide Mouse ELISPOT Ready-Set-Go! Sets & Antibody Selection Quick Guide
Intracellular Immunofluorescent Staining for Flow Cytometry
Brefeldin A BVD6-24G2 , 11B11 IL-5 NEW! mouse splenic CD4 ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hr
Neutralizing Bioassay Protocols
describe in vitro bioassays using neutralizing anti-mouse and anti-human cytokine antibodies. In general, the cytokine bioassay protocols are modified to pre-incubate the cytokine of interest with the specific neutralizing antibody prior to addition
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