- ￥1462.50
- 华韵生物(Huayunbio)
- HFC3599-20Tests
- 2025年07月13日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
20Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production.
|
| Alternate Names |
CSIF;Cytokine synthesis inhibitory factor;IL-10;Interleukin-10
|
| Swissprot |
P22301
|
| Gene ID |
3586
|
| Clone No |
JES3-9D7
|
| Host |
Rat
|
| Reactivity |
Human
|
| Application |
ICFCM
|
| Isotype |
Rat IgG1, κ
|
| Isotype Control |
FITC Rat IgG1, κ Isotype Control[HRPN]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
of 2 N HCl x30'. Wash 3x in PBS. Block in 1 mL 10% Horse serum, x30' at room temp. Wash 1x in PBS. Add 0.3 mL Biotinylated anti-BrdU (1:300, with 1% horse serum in PBS). Incubate at 4ºC o.n. or 2 hr. at room temp. Wash 3x in PBS.
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
FITC荧光二抗？――EarthOx新型DyLight 488绿色荧光
和1:400，即100μl和500μl的FITC标记山羊抗小鼠IgG二抗（货号为E031210）最终使用液是 10ml和50ml，而100μl和500μl的Dylight 488标记山羊抗小鼠IgG二抗（货号为E032210）最终使用液是40ml和200ml，可见，即使不考虑Dylight更高的荧光强度、更好的光学 稳定性和特异性，但就价格来说，Dylight荧光二抗也具有更高的性价比。下图为EarthOx原装进口DyLight 488标记，针对不同种属的二抗产品，艾美捷均提供现货包装：
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