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流式抗体/ FITC Anti-Mouse IL-10 An

tibody[JES5-16E3],HFC3596-25μg
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  • ¥1170
  • 华韵生物(Huayunbio)
  • HFC3596-25μg
  • 2025年07月15日
  • FCM
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保质期

      12个月

    • 供应商

      广州华韵生物科技有限公司

    • 应用范围

      FCM

    • 规格

      25μg

    温馨提示:产品由于优化升级,可能会增删改替。内容请以实时说明书信息为准。
    请联系华韵客服获取说明书。

    产品简介:

     

    Background
    L-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.
    Alternate Names
    CSIF;Cytokine synthesis inhibitory factor;IL-10;Interleukin-10
    Swissprot
    P18893
    Gene ID
    16153
    Clone No
    JES5-16E3
    Host
    Rat
    Reactivity
    Mouse
    Application
    ICFCM
    Isotype
    Rat IgG2b, κ
    Isotype Control
    FITC Rat IgG2b, κ Isotype Control[LTF-2]
    Conjugation
    FITC
    Conjugation Information
    FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
    Spectrum
    产品细节图片1
    Storage Buffer
    Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
    Storage
    This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
    Expiration date
    12个月
    Shipping
    冰袋

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    图标文献和实验
    相关实验
    • Protocol for intracytoplasmic staining of cytokines for FACS analysis

      ; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide

    • Dual- and Triple-Color Fluorospot

      in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb

    • Anti-GFP/BrdU In Situ Immunostaining

      of 2 N HCl x30'.  Wash 3x in PBS. Block in 1 mL 10% Horse serum, x30' at room temp.  Wash 1x in PBS. Add 0.3 mL Biotinylated anti-BrdU (1:300, with 1% horse serum in PBS).  Incubate at 4ºC o.n. or 2 hr. at room temp.  Wash 3x in PBS.

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