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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
20Tests
请联系华韵客服获取说明书。
产品简介:
| Background |
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
|
| Alternate Names |
IFN-gamma;IFNG;IFNγ;Interferon gamma
|
| Swissprot |
P01579
|
| Gene ID |
3458
|
| Clone No |
B27
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
ICFCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
APC Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
APC
|
| Conjugation Information |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
supernatant. [OPTIONAL] Block with 2% (2 µl) normal rat serum in 1X Permeabilization Buffer, in approximately 100 µl volume, at 4°C for 15 minutes. Without washing after blocking step, add 20 µl fluorochrome conjugated anti-human Foxp3 antibody
剂,重悬在流式染色缓冲液或者 PBS 里面)。如果需要长时间保存样本,建议在样本做完表面染色和固定后,保存在专门的保存缓冲液中。但即便是有专门的保存 buffer,对于用了串联染料(如 APC/Cy7、PE/Cy7 等)染色的样本,我们还是建议不要长时间保存,很难避免补偿的「漂移(shift)」。左图:human PBMC 刺激完当天染色后上机检测;右图:同一样本,表染和固定后,保存在 Cyto-Last™ buffer 里面,14 天后做胞内染色并上机。Ki- 67 染色时,选择哪种固定方式?流式
染色抗体(如 APC IFN-γ抗体),4℃ 孵育 30 min 或室温避光孵育 15 min。ICS Wash Buffe 液洗涤,1 500 rpm 离心 5 min。4. 弃离心上清液,根据实际情况加入 200 μL 或者 300 μL Wash Buffer 液重悬。5. 震荡混匀,流式上机。四. 注意事项1. 每一步离心倒掉上清时可以先在实验台上铺几层平板纸,倾倒上清后可吸掉管口的液体。2. 为了避免打孔剂对细胞形态的影响从而影响后续圈门影响实验结果,所以不管是否需要胞内染色,每个样本都需要打孔
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