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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
25μg
请联系华韵客服获取说明书。
产品简介:
| Background |
Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.
|
| Alternate Names |
Ly-6A.2/Ly-6E.1;Ly6;Ly6a;SCA-1;TAP
|
| Swissprot |
P05533
|
| Gene ID |
110454
|
| Clone No |
D7
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
Elab Fluor® 488 Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
Elab Fluor488
|
| Conjugation Information |
Elab Fluor 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔: Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4412 Anti-Rabbit IgG(H+L), F(ab
_blank()) + geom_hline(aes(yintercept = 0), data1,alpha=0.2) #设置主题+ labs(fill="Fold change") +ylab("Picture of text") #坐标标题+scale_x_discrete(breaks = c("A","B","C","D","E","F"),labels = c("D0","D1,"D2,"D3,"D4,"D5)) #更换刻度文本+ expand_limits(y=-300) #扩大
the probe stay for 1.5 hrs RT for complete erythrocyte lysis Count platelets under a phase contrast microscope at x400 magnification using Neubauer hemocytometer SOLUTIONS The Unopette Reservoir
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