- ￥1267.50
- 华韵生物(Huayunbio)
- HFC3470-50Tests
- 2025年07月14日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
50Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.
|
| Alternate Names |
AIM;CLEC2C;EA1;GP32/28;MLR-3;VEA
|
| Swissprot |
P37217
|
| Gene ID |
12515
|
| Clone No |
H1.2F3
|
| Host |
Armenian Hamster
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Armenian Hamster IgG
|
| Isotype Control |
FITC Armenian Hamster IgG Isotype Control[PIP]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
16/32（eBioscience， 目录号14-0161-81），在大鼠可以用纯化的抗大鼠的CD32，在人可以用过量的同种无关纯化Ig或血清。5、荧光素的选择检测相对低表达细胞因子如IL-4时，应选用PE或APC标记；单检测某一细胞因子时最好也选用PE或APC标记；同时检测多种细胞因子时，弱表达的应选用PE或APC，FITC标记最好用于高表达细胞因子如IFN-γ。八、问题与解答问题原因解决注释无CD69胞内染色细胞未激活激活剂制备不当，见激活剂制备储存一节。PMA Ionomycin激活4小时后CD
或APC标记;同时检测多种细胞因子时，弱表达的应选用PE或APC，FITC标记最好用于高表达细胞因子如IFN-γ。 八、问题与解答 问题 原因 解决 注释 无CD69胞内染色 细胞未激活 激活剂制备不当，见激活剂制备储存一节。 PMA Ionomycin激活4小时后CD3 T淋巴细胞CD69阳性率应>90% 使用了错误的抗凝剂 应使用肝素钠，不要使用肝素
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