- ￥624
- 华韵生物(Huayunbio)
- HFC2916-25μg
- 2025年07月14日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
25μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
|
| Alternate Names |
LCA;Ly-5.2
|
| Gene ID |
19264
|
| Clone No |
104.2
|
| Host |
Mouse
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
FITC Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验分泌。 随后进行表面染色：先以Fc阻断剂（2.4G2克隆，5 μg/mL）处理10分钟，再于4°C避光孵育15分钟，使用含1% BSA和0.1% NaN₃的DPBS缓冲液配制抗体，常用组合包括CD90.2、CD4、CD8、CD25、CD11b和CD45.2等标记。 固定步骤采用2%甲醛（FA，由含<3%甲醇的4% Roti-Histofix稀释），室温孵育40–60分钟。破膜与胞内染色使用0.1%皂苷缓冲液或1×Perm缓冲液，加入抗FoxP3、T-bet、RORγt、IL-17A或IFN-γ等抗体，4°C
注：此后步骤应注意避光操作。 1.2 滴加一抗 将4 μl Anti-DIG monoclonal antibody 和0.5 μl Texas-red-Avidin 稀释于100 μl 抗体稀释液1中，混匀；每张玻片滴加25 μl 于杂交区域，盖上22mm×22mm盖玻片，于37℃湿盒中孵育1小时；室温下4×SSC/0.1 % Tween-20中振荡洗涤5min×2缸。 1.3 滴加二抗
Purification of Endotoxin Free mAbs
electrophoresis will be the monoclonal antibody. In this case we normally estimate antibody concentration simply by the OD280/1.4 value. This material is suitable for most purposes, including in vitro conjugation to FITC or biotin, or for experimental use in vivo
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