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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
25μg
请联系华韵客服获取说明书。
产品简介:
| Background |
CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen.
|
| Alternate Names |
CD18;Cell surface adhesion glycoproteins LFA-1/CR3/p150+95 subunit beta;Complement receptor C3 subunit beta;Integrin beta-2;Itgb2
|
| Swissprot |
P11835
|
| Gene ID |
16414
|
| Clone No |
M18/2
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
Purified Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD18
Methods for the Detection of D-Amino-Acid Oxidase
crosslinker, Stratagene, La Jolla, CA). The membrane was incubated at 65°C for 1 hr in a prehybridization solution [5 x SSPE (1 x SSPE is 0.18 M NaCl, 10 mM sodium phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution (8 ), and 0.5% SDS] containing 20 μg
Methods for the Detection of D-Amino-Acid Oxidase
phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution ( 8 ), and 0.5% SDS] containing 20 μg/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham
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