- ￥312
- 华韵生物(Huayunbio)
- HFC2715-20Tests
- 2025年07月13日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
20Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
|
| Alternate Names |
CD8A;MAL;T-cell surface glycoprotein CD8 alpha chain;T-lymphocyte differentiation antigen T8/Leu-2
|
| Swissprot |
P01732
|
| Gene ID |
925
|
| Clone No |
OKT-8
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
PE Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验避光染色30分钟至过夜。 最后用相同破膜缓冲液洗涤两次，重悬后通过流式细胞仪采集数据。该方案兼顾报告荧光稳定性与核/胞质蛋白染色效率，适用于多参数精细免疫表型分析。 菲恩生物提供专业流式配色设计与高质检测试剂，优化多色方案，降低干扰，提升检测精准度，助力免疫细胞精细分选，赋能免疫学、肿瘤免疫及自身免疫疾病研究。欢迎合作，共促创新！ 以下是菲恩生物推荐的流式抗体搭配方案 种属 细胞群 流式抗体搭配 货号 Human T/B/NK细胞群检测 CD
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
Intracellular Cytokine Staining Protocol
, it is common to block the directly-conjugated anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking. eBioscience antibodies are available as Functional Grade (FG) Purified (sterile
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