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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
25μg
请联系华韵客服获取说明书。
产品简介:
| Background |
CD4, also known as T4, is a 55kD glycoprotein member of the immunoglobin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.
|
| Alternate Names |
CD4;T-cell surface antigen T4/Leu-3;T-cell surface glycoprotein CD4
|
| Swissprot |
P05540
|
| Gene ID |
24932
|
| Clone No |
OX-38
|
| Host |
Mouse
|
| Reactivity |
Rat
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
PE Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Mouse anti-Human HLA-DR mAb,PE 50T/100T 1000/1500 L0169 Rat anti-Mouse CD4 mAb, FITC 50T/100T 800/1200 L0170 Rat anti Mouse CD4 mAb, PE
,表现出Th1、Th2和TFH等具有传统功能的细胞亚群。导致Foxp3下调的关键因素包括高水平的炎症因子内环境,如可诱导效应性T细胞产生的细胞因子:IL-6和IFN-gamma。Tregs 细胞中Foxp3的表达分析CD4+CD25+ Treg 细胞中Foxp3 +细胞群表明与Foxp3+Treg完全重合。使用Foxp3鉴定Treg用Anti-Mouse CD4 FITC、Anti-Mouse CD25 APC和Anti-Mouse/Rat Foxp3 PE 标记BALB/c小鼠 脾细胞. 右上
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
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