- ￥208
- 华韵生物(Huayunbio)
- HFC2593-25μg
- 2025年07月09日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
25μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
|
| Alternate Names |
CD3E;CD3e;T-cell surface antigen T3/Leu-4 epsilon chain;T-cell surface glycoprotein CD3 epsilon chain;T3E
|
| Swissprot |
P22646
|
| Gene ID |
12501
|
| Clone No |
145-2C11
|
| Host |
Armenian Hamster
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Armenian Hamster IgG
|
| Isotype Control |
Purified Armenian Hamster IgG Isotype Control[PIP]
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
volume of Dynabeads taken from the vial (step 2). 2. Activation of Mouse T Cells 1) Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2) Add 2 µl Dynabeads Mouse T-Activator CD3/CD
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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