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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
25μg
请联系华韵客服获取说明书。
产品简介:
| Background |
CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.
|
| Alternate Names |
L3T4;T-cell surface antigen T4/Leu-3;T-cell surface glycoprotein CD4;T4
|
| Swissprot |
P06332
|
| Gene ID |
12504
|
| Clone No |
GK1.5
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
PerCP Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
PerCP
|
| Conjugation Information |
PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Intracellular Cytokine Staining Protocol
mature and developing T cells GK1.5 14-0041 T regulatory, CD4+CD25+ CD25 PC61.5 14-0251
Intracellular Cytokine Staining Protocol
GK1.5 14-0041 T regulatory, CD4+CD25+ CD25 PC61.5 14-0251 Natural T, NK+ NK1.1 PK136
对照应该是APC标记的Mouse IgG1,κ,货号是555751。注意同型对照的成份跟它的名称是相同的.如果是抗体的组合形式是纯化的一抗+荧光标记的二抗,那么应该选择一抗的同型对照。比如CDw125的纯化抗体,货号是555901,成分是Mouse IgG1,κ。它的同型对照是纯化的Mouse IgG1,κ,货号是555746。它的二抗PE标记的抗小鼠IgG1,货号是550083。那么染色方式如下: 样本管:纯化的CDw25+PE标记的抗Mouse IgG1+
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