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文献和实验Testing Rabbit Bleeds By Elisa
and rinse wells 4Xs with TBST. Rinse wells 2Xs with 50 mM HCO3; 0.5 mM MgCl2, pH 10. Develop in 1 mg/ml p-Nitrophenyl phosphate in 50 mM HCO3; 0.5 mM MgCl2, pH 10 (50 ul/well). Read A410 in ELISA reader.
Testing Rabbit Bleeds By Elisa
Testing Rabbit Bleeds By Elisa Claire Walczak January 1996 You can test whether or not you have gotten an immune response to the peptide and how strong that immune response is by doing ELISAs against peptide conjugated to BSA
Methods for the Detection of D-Amino-Acid Oxidase
then twice for 5 min. It was incubated for 1 hr in TBS-T containing rabbit anti-hog D-amino-acid oxidase IgG (1/3,000 dilution) and quickly rinsed twice with TBS-T and further washed in TBS-T once for 15 min then twice for 5 min. The membrane was incubated
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